1. High-Resolution Scientific-Grade Cooled Camera
Equipped with an approximately 6-megapixel, 16-bit scientific-grade cooled camera, delivering richer spatial detail while maintaining high sensitivity. Deep cooling suppresses background noise during long-exposure imaging, meeting the imaging requirements of both BLI and FLI modes.
2. Bioluminescence Imaging Module
Combines a fully darkened environment with a high-throughput lens to detect faint photon signals generated by luciferase reactions. Supports adjustable exposure time, pixel binning and lens aperture settings to accommodate experimental conditions ranging from strong signals to low-expression models.
3. Multi-Band Fluorescence Excitation Module
Features 20 monochromatic LED light sources across 24 excitation source positions, along with up to 18 narrow-band emission filters of different wavelengths, covering the visible to near-infrared-I (NIR-I) range, enabling fluorescence detection of a wide variety of fluorescent proteins and fluorescent probe labels. Each channel can be independently controlled by software, with compatibility for GFP, RFP, Cy5, Cy7, ICG and other commonly used fluorescent labels.
4. Motorized Emission Filter System
A 24-position motorized filter wheel automatically switches emission channels according to the experimental protocol. Narrow-band filters reduce excitation light leakage and crosstalk between different fluorescence signals, providing a foundation for multichannel imaging and spectral analysis.
5. Spectral Unmixing and Background Subtraction
Software separates target signals, autofluorescence and signals from multiple labels based on their spectral characteristics, improving image contrast when tissue autofluorescence is strong or probe spectra overlap.
6. Motorized Stage and High-Throughput Imaging
The motorized stage supports multiple field-of-view settings, autofocus and animal position adjustment, enabling local imaging of a single animal as well as batch imaging of up to 5 mice or 3 rats.
7. Animal Anesthesia and Temperature Maintenance
Integrates a temperature-controlled animal platform, multi-channel anesthesia nose cones and a waste-gas recovery interface, keeping animals at relatively consistent body temperature, posture and anesthesia state across multiple imaging time points.
8. Integrated Analysis Software
A single software platform handles BLI and FLI image acquisition, background correction, image overlay and quantitative analysis. Supports batch ROI replication, inter-group comparison, time-intensity curves and raw data export.
